Human CaM Kinase II delta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4176
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CaM Kinase II delta
Ala402-Lys478
Accession # Q13557
Ala402-Lys478
Accession # Q13557
Specificity
Detects human CaM Kinase II delta isoforms. No cross-reactivity with recombinant human CaM Kinase II alpha, CaM Kinase II beta, or CaM Kinase II gamma is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CaM Kinase II delta Antibody
Detection of Human CaM Kinase II delta by Western Blot.
Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, K562 human chronic myelogenous leukemia cell line, MDA-MB-468 human breast cancer cell line, and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human CaM Kinase IId Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaM Kinase IId at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CaM Kinase II delta in Human Brain.
CaM Kinase IId was detected in immersion fixed paraffin-embedded sections of human brain (cerebral cortex) using 15 µg/mL Sheep Anti-Human CaM Kinase IId Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4176) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human CaM Kinase II delta Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebral cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebral cortex)
Western Blot
2 µg/mL
Sample: SW480 human colorectal adenocarcinoma cell line, K562 human chronic myelogenous leukemia cell line, MDA-MB-468 human breast cancer cell line, and SK-Mel-28 human malignant melanoma cell line
Sample: SW480 human colorectal adenocarcinoma cell line, K562 human chronic myelogenous leukemia cell line, MDA-MB-468 human breast cancer cell line, and SK-Mel-28 human malignant melanoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CaM Kinase II delta
Calcium/calmodulin-dependent protein kinase type II (CaMKII) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and delta, and each can yield several isoforms through alternative splicing. Each CaMKII protein assembles into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMKII delta expression is ubiquitous, and has been identified as the predominant isozyme in the heart, where it is implicated in the development of cardiac hypertrophy and heart failure.
Long Name
Calcium/calmodulin-dependent Protein Kinase II delta Subunit
Alternate Names
CAMK2D
Gene Symbol
CAMK2D
UniProt
Additional CaM Kinase II delta Products
Product Documents for Human CaM Kinase II delta Antibody
Product Specific Notices for Human CaM Kinase II delta Antibody
For research use only
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