Human/Canine IL-23 p19 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6250
Key Product Details
Species Reactivity
Human, Canine
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant canine IL-23 p19
Arg23-Pro193
Accession # XP_538231
Arg23-Pro193
Accession # XP_538231
Specificity
Detects human and canine IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant feline IL-23 p19 is observed and approximately 40% cross-reactivity with recombinant mouse IL-23 p19 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Canine IL-23 p19 Antibody
Detection of Human and Canine IL‑23 p19 by Western Blot.
Western blot shows lysates of human mature dendritic cells (LPS activated), CLL-1390 canine leukocytic round cell neoplasia cell line, and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Canine IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6250) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-23 p19 at approximately 26-28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human/Canine IL-23 p19 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Human mature dendritic cells (LPS activated), CLL‑1390 canine leukocytic round cell neoplasia cell line, and THP‑1 human acute monocytic leukemia cell line
Sample: Human mature dendritic cells (LPS activated), CLL‑1390 canine leukocytic round cell neoplasia cell line, and THP‑1 human acute monocytic leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-23
IL-23 p19 (Interleukin 23 p19; also IL-23 alpha) is an 18-20 kDa (estimated) member of the IL-6 superfamily of molecules. It is disulfide-bonded to p40 to form IL-23. IL-23 is secreted by immune-related cell types such as keratinocytes, dendritic cells, macrophages, microglia and monocytes, often following TLR stimulation. It appears to drive Th17 cell development by inhibiting T-bet and FoxP3 production, and to reduce IL-12-mediated IFN-gamma production by CD4+, CD8+ and NK cells. Based on human, mature canine IL-23 p19 is 171 amino acids (aa) in length. It is a alpha-helical molecule that utilizes Cys77 to form an interchain disulfide bond with IL-12 p40. IL-23 p19 does not appear to be released unless dimerized to p40. There are no potential N-linked glycosylation sites on p19. Mature canine IL-23 p19 (aa 23-193) shares 88% aa identity with human IL-23 p19.
Long Name
Interleukin 23
Alternate Names
IL-23 p19/IL-12 p40, IL-23p19/IL-12p40, IL23, SGRF
Gene Symbol
IL23A
UniProt
Additional IL-23 Products
Product Documents for Human/Canine IL-23 p19 Antibody
Product Specific Notices for Human/Canine IL-23 p19 Antibody
For research use only
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