Human Carbonic Anhydrase I/CA1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2180
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Carbonic Anhydrase I/CA1
Ala2-Phe261
Accession # P00915
Ala2-Phe261
Accession # P00915
Specificity
Detects human Carbonic Anhydrase I/CA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) CA2, rhCA3, rhCA4, rhCA8, rhCA9, rhCA10, and rhCA12 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Carbonic Anhydrase I/CA1 Antibody
Detection of Human Carbonic Anhydrase I/CA1 by Western Blot.
Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, human liver tissue, and human colon tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Carbonic Anhydrase I/CA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Carbonic Anhydrase I/CA1 by Simple WesternTM.
Simple Western lane view shows lysates of HEL 92.1.7 human erythroleukemic cell line and human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 38 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Carbonic Anhydrase I/CA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2180) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Carbonic Anhydrase I/CA1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase I (Catalog # 2180-CA), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase I (Catalog # 2180-CA), see our available Western blot detection antibodies
Simple Western
5 µg/mL
Sample: HEL 92.1.7 human erythroleukemic cell line and human liver tissue
Sample: HEL 92.1.7 human erythroleukemic cell line and human liver tissue
Western Blot
0.1 µg/mL
Sample: HEL 92.1.7 human erythroleukemic cell line, human liver tissue, and human colon tissue
Sample: HEL 92.1.7 human erythroleukemic cell line, human liver tissue, and human colon tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase I/CA1
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation (2). The activity of CA1 can also be measured by its ability to catalyze the reaction CO2 + H2O → HCO3- + H+, using a published method (3).
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.
- Wilbur, K.M. and N.G. Anderson (1948) J. Biol. Chem. 176:147.
Alternate Names
CA1
Entrez Gene IDs
759 (Human)
Gene Symbol
CA1
UniProt
Additional Carbonic Anhydrase I/CA1 Products
Product Documents for Human Carbonic Anhydrase I/CA1 Antibody
Product Specific Notices for Human Carbonic Anhydrase I/CA1 Antibody
For research use only
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