Human Carbonic Anhydrase IX/CA9 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB21881
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 303104
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX/CA9
Pro59-Asp414
Accession # Q16790
Pro59-Asp414
Accession # Q16790
Specificity
Detects human Carbonic Anhydrase IX/CA9 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Carbonic Anhydrase IX/CA9 Antibody
Carbonic Anhydrase IX/CA9 in Human Colon.
Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB21881) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Carbonic Anhydrase IX/CA9 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon
Sample: Immersion fixed paraffin-embedded sections of human colon
Reviewed Applications
Read 2 reviews rated 4.5 using MAB21881 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase IX/CA9
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1‑3). CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)‑associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). The immunogen corresponds to the extracellular portion of human CA9.
References
- Pastorek, J. et al. (1994) Oncogene 9: 2877.
- Opavsky, R. et al. (1996) Genomics 33: 480.
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
- Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Alternate Names
CA9, G250, MN, RCC
Gene Symbol
CA9
UniProt
Additional Carbonic Anhydrase IX/CA9 Products
Product Documents for Human Carbonic Anhydrase IX/CA9 Antibody
Product Specific Notices for Human Carbonic Anhydrase IX/CA9 Antibody
For research use only
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