Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5388
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 487908
Product Summary for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody
Immunogen
E. coli-derived recombinant human Carbonic Anhydrase VA/VB (CA5A/5B)
Ala40-Ser305
Accession # P35218
Ala40-Ser305
Accession # P35218
Specificity
Detects human Carbonic Anhydrase VA/VB (CA5A/5B) in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CA1, 4, or CA6‑14 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody
Carbonic Anhydrase VA/VB (CA5A/5B) in Human Liver.
Carbonic Anhydrase VA/VB (CA5A/5B) was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Carbonic Anhydrase VA/ VB (CA5A/5B) Monoclonal Antibody (Catalog # MAB5388) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Carbonic Anhydrase VA (Catalog # 3049-CA)
Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA)
Sample: Recombinant Human Carbonic Anhydrase VA (Catalog # 3049-CA)
Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase VA/VB (CA5A/5B)
Carbonic Anhydrase is a group of metalloproteases that catalyze the reversible reaction of CO2 + H2O → HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA5A and CA5B are homologous proteins that are encoded by different genes locating in different chromosomes. However, they both are mitochondrial proteins and have low Carbonic Anhydrase activity. The amino acid sequence of human CA5A is 98%, 80% and 76% identical to that of chimpanzee, dog and mouse.
Alternate Names
CA5;CA5AD;Carbonic anhydrase 5A, mitochondrial;CAV;CAVA;GS1-21A4.1
Gene Symbol
CA5A
UniProt
Additional Carbonic Anhydrase VA/VB (CA5A/5B) Products
Product Documents for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody
Product Specific Notices for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody
For research use only
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