Human Carbonic Anhydrase VI Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2939
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase VI
Gln18-Asn308
Accession # EAW71606
Gln18-Asn308
Accession # EAW71606
Specificity
Detects human Carbonic Anhydrase VI in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Carbonic Anhydrase (CA) 5A and rhCA5B is observed and less than 1% cross-reactivity with rhCA1, rhCA2, rhCA3, rhCA4, rhCA7, rhCA8, rhCA9, rhCA10, rhCA12, rhCA13, and rhCA14 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Carbonic Anhydrase VI Antibody
Detection of Human Carbonic Anhydrase VI by Western Blot.
Western blot shows human saliva, human milk, and lysates of HSC-3 human oral squamous cell carcinoma cell line and LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Carbonic Anhydrase VI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2939) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase VI at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Carbonic Anhydrase VI by Simple WesternTM.
Simple Western lane view shows lysates of human saliva, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase VI at approximately 56 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Carbonic Anhydrase VI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2939) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Carbonic Anhydrase VI Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VI (Catalog # 2939-CA), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VI (Catalog # 2939-CA), see our available Western blot detection antibodies
Simple Western
1 µg/mL
Sample: Human saliva
Sample: Human saliva
Western Blot
0.5 µg/mL
Sample: Human saliva, human milk, HSC-3 human oral squamous cell carcinoma cell line, and LoVo human colorectal adenocarcinoma cell line
Sample: Human saliva, human milk, HSC-3 human oral squamous cell carcinoma cell line, and LoVo human colorectal adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase VI
Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc-metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5).
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Murakami, H. and Sly, W. S. (1987) J. Biol. Chem. 262:1382.
- Thatcher, B. J. et al. (1998) Biochem. Biophys. Res. Commun. 250:635.
- Hankin, R. I. et al. (1999) Am. J. Med. Sci. 318:380.
- Karhumaa, P. et al. (2001) Proc. Natl. Acad. Sci. USA. 98:11604.
Alternate Names
CA6, Gustin
Gene Symbol
CA6
UniProt
Additional Carbonic Anhydrase VI Products
Product Documents for Human Carbonic Anhydrase VI Antibody
Product Specific Notices for Human Carbonic Anhydrase VI Antibody
For research use only
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