Human Carbonic Anhydrase XIII/CA13 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2194
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Carbonic Anhydrase XIII/CA13
Ser2-His262
Accession # Q8N1Q1
Ser2-His262
Accession # Q8N1Q1
Specificity
Detects human Carbonic Anhydrase XIII/CA13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant human (rh) CA1, rhCA2, and rhCA3 is observed and 5% cross‑reactivity with rhCA4, rhCA8, rhCA9, rhCA10, rhCA12, and rhCA14 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Carbonic Anhydrase XIII/CA13 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase XIII (Catalog # 2194-CA), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase XIII (Catalog # 2194-CA), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Carbonic Anhydrase XIII (Catalog # 2194-CA)
Sample: Recombinant Human Carbonic Anhydrase XIII (Catalog # 2194-CA)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase XIII/CA13
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA13 is a cytosolic enzyme with a unique and widespread distribution pattern as compared to the other cytosolic CAs, indicating that it may play important physiological roles in several organs (2).
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Lehtonen, J. et al. (2003) J. Biol. Chem. 279: 2719.
Alternate Names
CA13
Entrez Gene IDs
377677 (Human)
Gene Symbol
CA13
UniProt
Additional Carbonic Anhydrase XIII/CA13 Products
Product Documents for Human Carbonic Anhydrase XIII/CA13 Antibody
Product Specific Notices for Human Carbonic Anhydrase XIII/CA13 Antibody
For research use only
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