Human CARD9 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5248
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CARD9
Lys225-Gln359
Accession # Q9H257
Lys225-Gln359
Accession # Q9H257
Specificity
Detects human CARD9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) CARD6, rhCARD8, rhCARD11, rhCARD14, and rhCARD10 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CARD9 Antibody
Detection of Human CARD9 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CARD9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5248) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CARD9 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human CARD9 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CARD9
CARD9 (caspase recruitment domain-containing protein 9) is a 70 kDa member of the CARD module family of proteins. It is expressed in myeloid lineage cells such as macrophages, neutrophils and dendritic cells and mediates activation of p38 and Jnk, as well as NF-kappa B by interacting with BCL10 and MALT1 in a PKC-independent manner. Human CARD9 is 536 amino acids (aa) in length. It contains one CARD domain (aa 6‑98), two coiled-coil regions (aa 117‑277 and 332‑419) and a phosphorylation site at Ser460. CARD9 dimerizes with itself and BCL10. Multiple potential splice variants are noted for CARD9. There are 35 aa and 39 aa substitutions for aa 479‑536, a seven aa substitution for aa 360‑536 and an eleven aa substitution for aa 482‑536. Over aa 9‑256, human CARD9 is 88% aa identical to mouse CARD9.
Long Name
Caspase Recruitment Domain Containing Protein 9
Alternate Names
CANDF2, caspase recruitment domain family, member 9, caspase recruitment domain-containing protein 9, hCARD9
Gene Symbol
CARD9
UniProt
Additional CARD9 Products
Product Documents for Human CARD9 Antibody
Product Specific Notices for Human CARD9 Antibody
For research use only
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