Human CARF Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4195
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CARF
Ser379-Leu579
Accession # Q9NXV6
Ser379-Leu579
Accession # Q9NXV6
Specificity
Detects human CARF in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CARF Antibody
Detection of Human CARF by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, SK-Mel-28 human malignant melanoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Human CARF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4195) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CARF at approximately 61 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human CARF Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line, SK-Mel-28 human malignant melanoma cell line, and U2OS human osteosarcoma cell line
Sample: HEK293 human embryonic kidney cell line, SK-Mel-28 human malignant melanoma cell line, and U2OS human osteosarcoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CARF
CARF (collaborator of ARF) is a component of the ARF-MDM2-p53 pathway. CARF interacts with ARF in the nucleolus accentuating ARF-mediated p53 stabilization and activation. Data also suggests that in the absence of ARF, CARF interacts directly with p53 in the nucleoplasm resulting in a moderate stabilization and activation of p53.
Long Name
Collaborates/Cooperates With ARF [Alternate Reading Frame] Protein
Alternate Names
CDKN2AIP
Gene Symbol
CDKN2AIP
UniProt
Additional CARF Products
Product Documents for Human CARF Antibody
Product Specific Notices for Human CARF Antibody
For research use only
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