Human Caspr1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7548

R&D Systems, part of Bio-Techne
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AF7548
AF7548-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Caspr1 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Caspr1
Trp20-Gly1280
Accession # P78357

Specificity

Detects human Caspr1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human Caspr2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Caspr1 Antibody

Caspr1 antibody in Human Brain by Immunohistochemistry (IHC-P).

Caspr1 in Human Brain.

Caspr1 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Caspr1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7548) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to paranodal axonal regions in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Caspr1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspr1

CASPR1 (Contactin-ASsociated PRotein-1; also Neurexin-4 and p190) is a 180-195 kDa member of the neurexin family of proteins. It is expressed on both axons and dendrites of neurons, and likely serves the critical function of directing the movement of low MW contactin, AMPA receptors, and Na+ plus K+ channels to appropriate sites on the cell membrane. This indicates participation in key processes such as myelination and excitatory neurotransmission. Mature human CASRP1 is a 1365 amino acid (aa) type I transmembrane protein. It contains a 1264 aa extracellular region (aa 20-1283) plus an 80 aa cytoplasmic domain. The extracellular region is highly modular, possessing one discoidin (carbohydrate-binding) domain (aa 24-158), four laminin G-like domains (aa 203-513, 813-956 and 1088-1250), two EGF-like domains (aa 540-577 and 957-996) and one fibrinogen C-terminal globular regions (aa 576-795). There is one potential alternative start site at Met507. Over aa 20-1280, human CASPR1 shares 94% aa sequence identity with mouse CASPR1.

Long Name

Contactin Associated Protein-like 1

Alternate Names

CNTNAP, CNTNAP1, p190

Entrez Gene IDs

8506 (Human); 53321 (Mouse)

Gene Symbol

CNTNAP1

UniProt

P78357 (Human)

Additional Caspr1 Products

Product Documents for Human Caspr1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Caspr1 Antibody

For research use only

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