Human Cathepsin O Antibody

Name: Human Cathepsin O Antibody
Brand: R&D Systems
SKU: AF1174

R&D Systems, part of Bio-Techne | Catalog # AF1174

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Cathepsin O Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin O
Asp24-Val321
Accession # P43234

Specificity

Detects human Cathepsin O in direct ELISAs and Western blots. In Western blots, less than 2% cross‑reactivity with recombinant human Cathepsin A, B, C, D, L, and Z is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Cathepsin O Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin O, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cathepsin O

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin O

The protein encoded by the Cathepsin O gene is a cysteine proteinase and a member of the papain superfamily. This proteolyticenzyme is involved in cellular protein degradation and turnover. The recombinant form of this enzyme was shown todegrade synthetic peptides typically used as substrates for cysteine proteinases and its proteolytic activity wasabolished by an inhibitor of cyteine proteinase. (provided by RefSeq)

Alternate Names

CTSO

Entrez Gene IDs

1519 (Human)

Gene Symbol