Human Cathepsin S Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1183
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Surface Plasmon Resonance
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 248718
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
Gln17-Ile331 (predicted)
Accession # P25774
Gln17-Ile331 (predicted)
Accession # P25774
Specificity
Detects pro and mature forms of human Cathepsin S in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Cathepsin B, C, D, L, L2/V, X/Z/P, recombinant mouse Cathepsin F, or H is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for Human Cathepsin S Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin S (Catalog # 1183-CY), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin S (Catalog # 1183-CY), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Cathepsin S (Catalog # 1183-CY)
Sample: Recombinant Human Cathepsin S (Catalog # 1183-CY)
Reviewed Applications
Read 3 reviews rated 3.7 using MAB1183 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin S
Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1‑16), a pro region (residues 17‑114), and the mature enzyme (residues 115‑331) (3‑5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages and other phagocytic cells.
References
- Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104, Academic Press, San Diego.
- Turk, V. et al. (2001) EMBO J. 20:4629.
- Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
- Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
- Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.
Alternate Names
CTSS
Entrez Gene IDs
1520 (Human)
Gene Symbol
CTSS
UniProt
Additional Cathepsin S Products
Product Documents for Human Cathepsin S Antibody
Product Specific Notices for Human Cathepsin S Antibody
For research use only
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