Human Cathepsin V Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1080
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin V
Val18-Val334
Accession # O60911
Val18-Val334
Accession # O60911
Specificity
Detects human Cathepsin V in Western blots. In this format, approximately 15% cross-reactivity with recombinant human (rh) Cathepsin C, rhCathepsin A, rhCathepsin D, rhCathepsin O is observed and less than 5% cross-reactivity with rhCathepsin S and rhCathepsin X/Z/P is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Cathepsin V Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Cathepsin V (Catalog # 1080-CY)
Sample: Recombinant Human Cathepsin V (Catalog # 1080-CY)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin V
Cathepsin V (also known as Cathepsin L2 and U) is a cysteine protease of the papain family (1). It is expressed as a proenzyme in lysosomes, which can be autocatalytically converted to the mature form at pH 4.0 (2). Cathepsin V shows high amino acid identity with Cathepsin L (77% for the proenzymes and 80% for the mature enzymes) (3). However, Cathepsin V is specifically expressed in the thymus, testis, and corneal epithelium whereas Cathepsin L is expressed throughout the body.
References
- Turk, V. et al. (2001) EMBO J. 20:4629.
- Bromme, D. et al. (1999) Biochemistry 38:2377.
- Somoza, J.R. et al. (2000) Biochemistry 39:12543.
Alternate Names
Cathepsin L2, Cathepsin U, CTSL2
Entrez Gene IDs
1515 (Human)
Gene Symbol
CTSV
UniProt
Additional Cathepsin V Products
Product Documents for Human Cathepsin V Biotinylated Antibody
Product Specific Notices for Human Cathepsin V Biotinylated Antibody
For research use only
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