Human Cathepsin X/Z/P Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF934
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin X/Z/P
Ala21-Val303 (Gly23Val, Ser48Thr)
Accession # Q9UBR2
Ala21-Val303 (Gly23Val, Ser48Thr)
Accession # Q9UBR2
Specificity
Detects human Cathepzin X/Z/P in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) Cathepsin A, rhCathepsin B, rhCathepsin C, rhCathepsin D, and rhCathepsin L is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Cathepsin X/Z/P Biotinylated Antibody
Cathepsin X/Z/P in Human Breast Cancer Tissue.
Cathepsin X/Z/P was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Cathepsin X/Z/P Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF934) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Cathepsin X/Z/P Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Human Cathepsin X/Z/P (Catalog # 934-CY)
Sample: Recombinant Human Cathepsin X/Z/P (Catalog # 934-CY)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin X/Z/P
Cathepsin X (also known as Cathepsin Z and P) is a cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).
References
- Nagler, D.K. and R. Menard (1998) FEBS Lett. 434:135.
- Santamaria, I. et al. (1998) J. Biol. Chem. 273:16816.
- Deussing, J. et al. (2000) Biochim. Biophys. Acta 1491:93.
- Pungercar, J. and G. Ivanovski (2000) Pflugers Arch. Eur. J. Physiol. 439:R116.
- Pungercar, J. et al. (2000) Pflugers Arch. Eur. J. Physiol. 439:R119.
- Sivaraman, J. et al. (2000) J. Mol. Biol. 295:935.
- Menard, R. et al. (2001) Biol. Chem. 382:839.
- Therrien, C. et al. (2001) Biochemistry 40:2702.
- Klemencic, I. et al. (2000) Eur. J. Biochem. 267:5404.
- Guncar, G. et al. (2000) Structure Fold Des. 8:305.
- Lustigman, S. et al. (1996) J. Biol. Chem. 271:30181.
Alternate Names
Cathepsin P, Cathepsin Z, CTSZ
Gene Symbol
CTSZ
UniProt
Additional Cathepsin X/Z/P Products
Product Documents for Human Cathepsin X/Z/P Biotinylated Antibody
Product Specific Notices for Human Cathepsin X/Z/P Biotinylated Antibody
For research use only
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