Human CCK Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11040
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2753A
Product Specifications
Immunogen
Human CCK synthetic peptide
Accession # P06307
Accession # P06307
Specificity
Detects human CCK in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human CCK Antibody
CCK in Capan-2 Human Cell Line.
CCK was detected in immersion fixed Capan-2 human pancreatic adenocarcinoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Rabbit Anti-Human CCK Monoclonal Antibody (Catalog # MAB11040) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
CCK in Human Duodenum.
CCK was detected in immersion fixed paraffin-embedded sections of human duodenum using Rabbit Anti-Human CCK Monoclonal Antibody (Catalog # MAB11040) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuroendocrine cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human CCK Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line
Sample: Immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human duodenum
Sample: Immersion fixed paraffin-embedded sections of human duodenum
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCK
Cholecystokinin (CCK) is a peptide hormone secreted by the gastrointestinal system that stimulates the digestion of protein and fat. CCK is synthesized and secreted by enteroendocrine cells of the duodenum and acts as a hunger suppressant. CCK participates in several processes such as digestion, satiety, and anxiety. CCK is also found extensively throughout the central nervous system with anxiogenic, panicogenic and hallucinogenic effects. Receptors of CCK are found in the central nervous system specifically in the hippocampus, cerebral cortex, and striatum.
References
Lenka A., Arumugham
SS., Christopher R., Pal PK, "Genetic Substrates of Psychosis in Patients
with Parkinson's Disease: A Critical Review". Journal of the
Neurological Sciences. 2016 May; 364: 33-41.
Zwanzger P.,
Domschke K., Bradwejn J., "Neuronal Network of Panic Disorder: The Role of the
Neuropeptide Cholecystokinin". Depression and Anxiety. 2012 Sep. 29(9): 762-74.
Okonkwo O., Zezoff
D., Adeyinka A., "Biochemistry, Cholecystokinin". StatPearls. 2021 Jan. Available from: https://www.ncbi.nlm.nih.gov/books/NBK534204/.
Long Name
Cholecystokinin
Alternate Names
cholecystokinin, MGC117187
Entrez Gene IDs
885 (Human)
Gene Symbol
CCK
UniProt
Additional CCK Products
Product Documents for Human CCK Antibody
Product Specific Notices for Human CCK Antibody
For research use only
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