Human CCL1/I-309/TCA-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF272
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Neutralization, Western Blot
Cited:
Affinity Purification, Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CCL1/I‑309/TCA‑3
Lys24-Lys96
Accession # P22362
Lys24-Lys96
Accession # P22362
Specificity
Detects human CCL1/I‑309/TCA‑3 in direct ELISAs and Western blots. Neutralizes the biological activity of recombinant human CCL1. Does not block the biological activity of recombinant mouse TCA-3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.15 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL1/I-309/TCA-3 Antibody
Chemotaxis Induced by CCL1/I-309 and Neutralization by Human CCL1/I-309 Antibody.
Recombinant Human CCL1/I-309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I-309 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL1/I-309 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF272). The ND50 is typically 0.4-2.0 µg/mL.Applications for Human CCL1/I-309/TCA-3 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human CCL1/I-309/TCA-3 (Catalog # 272-I)
Sample: Recombinant Human CCL1/I-309/TCA-3 (Catalog # 272-I)
Neutralization
Measured by its ability to neutralize CCL1/I-309/TCA-3-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 0.02 µg/mL Recombinant Human CCL1/I-309/TCA-3.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL1/I-309/TCA-3
Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma/ delta T cell line but not in EBV‑transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.
CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15‑16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.
References
- Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci USA 89:2950.
- Miller, M.D. et al. (1990) J. Immunol. 145:2737.
Alternate Names
I-309, P500, SCYA1, SCYA2, TCA-3, TCA3
Gene Symbol
CCL1
UniProt
Additional CCL1/I-309/TCA-3 Products
Product Documents for Human CCL1/I-309/TCA-3 Antibody
Product Specific Notices for Human CCL1/I-309/TCA-3 Antibody
For research use only
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