Human CCL15/MIP-1 delta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB363
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Cited:
Antibody Array Development, Immunocytochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 52130
Product Specifications
Immunogen
E. coli-derived recombinant human CCL15/MIP-1 delta
Gln22-Ile113
Accession # Q16663.2
Gln22-Ile113
Accession # Q16663.2
Specificity
Detects human CCL15/MIP‑1 delta in ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL15/MIP-1 delta Antibody
Chemotaxis Induced by CCL15/MIP‑1 delta and Neutral-ization by Human CCL15/ MIP‑1 delta Antibody.
Recombinant Human CCL15/ MIP-1d (Catalog # 363-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/ MIP-1d (16 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL15/MIP-1d Mono-clonal Antibody (Catalog # MAB363). The ND50 is typically 3-15 µg/mL.Applications for Human CCL15/MIP-1 delta Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CCL15/MIP-1 delta 92 aa (Catalog # 363-MG)
Sample: Recombinant Human CCL15/MIP-1 delta 92 aa (Catalog # 363-MG)
Neutralization
Measured by its ability to neutralize CCL15/MIP-1 delta-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 16 ng/mL Recombinant Human CCL15/MIP-1 delta 92 aa.
Human CCL15/MIP-1 delta Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
Use in combination with these reagents:
- Detection Reagent: Human CCL15/MIP‑1 delta Biotinylated Antibody (Catalog # BAF363)
- Standard: Recombinant Human CCL15/MIP-1 delta/LKN-1 (92 aa) Protein (Catalog # 363-MG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL15/MIP-1 delta
CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1 delta is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-1 delta is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.
References
- Wang, W. et al. (1998) J. Clinical Immunol. 18:214
Alternate Names
HCC-2, Leukotactin 1, MIP-1 delta, MIP-5, MIP1 delta, NCC-3
Entrez Gene IDs
6359 (Human)
Gene Symbol
CCL15
UniProt
Additional CCL15/MIP-1 delta Products
Product Documents for Human CCL15/MIP-1 delta Antibody
Product Specific Notices for Human CCL15/MIP-1 delta Antibody
For research use only
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