Human CCL15/MIP-1 delta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3631
Key Product Details
Species Reactivity
Human
Applications
Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 88119
Product Specifications
Immunogen
E. coli-derived recombinant human CCL15/MIP-1 delta
Ser46-Ile113
Accession # Q16663
Ser46-Ile113
Accession # Q16663
Specificity
Detects human CCL15/MIP‑1 delta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human
CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,
recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 12, 17, 19, 20, 21, 22, 24,
25, or recombinant rat CCL20 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL15/MIP-1 delta Antibody
Chemotaxis Induced by CCL15/MIP‑1 delta and Neutral-ization by Human CCL15/ MIP‑1 delta Antibody.
Recombinant Human CCL15/ MIP-1d (Catalog # 628-LK) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/ MIP-1d (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL15/MIP-1d Monoclonal Antibody (Catalog # MAB3631). The ND50 is typically 5-20 µg/mL.Applications for Human CCL15/MIP-1 delta Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CCL15/MIP-1 delta 92 aa (Catalog # 363-MG)
Sample: Recombinant Human CCL15/MIP-1 delta 92 aa (Catalog # 363-MG)
Neutralization
Measured by its ability to neutralize CCL15/MIP‑1 delta-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 1 ng/mL Recombinant Human CCL15/MIP‑1 delta 68 aa.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL15/MIP-1 delta
CCL15, also called macrophage inflammatory protein 1 delta (MIP-1 delta and Leukotactin-1 (LKN-1), is a CC chemokine with two additional cysteine residues that form a third disulfide bond. CCL15 functions as a chemoattractant for monocytes, T cells, and eosinophils through interactions with CCR1. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1 delta is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells.
References
- Wang, W. et al. (1998) J. Clinical Immunol. 18:214.
Alternate Names
HCC-2, Leukotactin 1, MIP-1 delta, MIP-5, MIP1 delta, NCC-3
Entrez Gene IDs
6359 (Human)
Gene Symbol
CCL15
UniProt
Additional CCL15/MIP-1 delta Products
Product Documents for Human CCL15/MIP-1 delta Antibody
Product Specific Notices for Human CCL15/MIP-1 delta Antibody
For research use only
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