Human CCL25/TECK Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB334
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Neutralization
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 52513
Product Specifications
Immunogen
E. coli-derived recombinant human CCL25/TECK
Gln24-Leu150
Accession # AAB69981
Gln24-Leu150
Accession # AAB69981
Specificity
Detects human CCL25/TECK in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26,
recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9/10/MIP-1 gamma, 11, 12, 17, 19, 21, 22, or
recombinant rat CCL20.
is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL25/TECK Antibody
Chemotaxis Induced by CCL25/TECK and Neutral-ization by Human CCL25/TECK Antibody.
Recombinant Human CCL25/TECK (Catalog # 334-TK) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL25/TECK (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL25/ TECK Monoclonal Antibody (Catalog # MAB334). The ND50 is typically 3-12 µg/mL.Applications for Human CCL25/TECK Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize CCL25/TECK-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR9. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 1.5 µg/mL Recombinant Human CCL25/TECK.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL25/TECK
CCL25 (thymus-expressed chemokine) is a CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 aa precursor protein with a 23 aa signal peptide that is cleaved to yield a 127 aa mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 aa protein that exhibits 49% aa sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 are chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 is also a specific agonist for the CCR9 chemokine receptor
References
- Vicari, A.P. et al. (1997) Immunity 7:291.
Alternate Names
TECK
Gene Symbol
CCL25
UniProt
Additional CCL25/TECK Products
Product Documents for Human CCL25/TECK Antibody
Product Specific Notices for Human CCL25/TECK Antibody
For research use only
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