Human CCL3/CCL4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB270
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Neutralization, Western Blot
Cited:
Blocking, Co-Immunoprecipitation, ELISpot Development, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 93321
Product Specifications
Immunogen
E. coli-derived recombinant human CCL3/MIP-1 alpha
Ala27-Ala92
Accession # P10147
Ala27-Ala92
Accession # P10147
Specificity
Detects human CCL3/MIP-1 alpha in direct ELISAs and
Western blots. In direct ELISAs, greater than 75% cross-reactivity with
recombinant human (rh) CCL4/MIP-1 beta is observed while approximately 30%
cross-reactivity with recombinant mouse (rm) CCL3, rmCCL4/MIP-1 beta, and rhCCL14
is observed. No cross-reactivity with rhCCL1, 2, 5, 7, 8, 11, 13, 15, 16, 17,
18, 19, 20, 21, 22, 23, 24, 25, 26, rmCCL1, 2, 5, 6, 7, 9/10/MIP-1 gamma, 11, 12,
17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL3/CCL4 Antibody
CCL3/CCL4 in U266 Human Cell Line.
CCL3/CCL4 was detected in immersion fixed U266 human myeloma cell line (positive staining) and HL-60 human acute promyelocytic leukemia cell line (negative staining) using Mouse Anti-Human CCL3/CCL4 Monoclonal Antibody (Catalog # MAB270) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Human CCL3/CCL4 Antibody.
Recombinant Human CCL3/MIP-1a isoform LD78a (Catalog # 270-LD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL3/MIP-1a isoform LD78a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL3/CCL4 Monoclonal Antibody (Catalog # MAB270). The ND50 is typically 0.075-0.375 µg/mL.Applications for Human CCL3/CCL4 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed U266 human myeloma cell line
Sample: Immersion fixed U266 human myeloma cell line
Western Blot
1 µg/mL
Sample: Recombinant Human CCL3/MIP‑1 alpha isoform LD78a (Catalog # 270-LD) under non-reducing conditions only
Sample: Recombinant Human CCL3/MIP‑1 alpha isoform LD78a (Catalog # 270-LD) under non-reducing conditions only
Neutralization
Measured by its ability to neutralize CCL3/MIP-1 alpha-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.075-0.375 µg/mL in the presence of 10 ng/mL Recombinant Human CCL3/MIP‑1 alpha isoform LD78a.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/CCL4
References
- Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
Long Name
CCL3/MIP-1 alpha and CCL4/MIP-1 beta Cross-reactive
Alternate Names
C-C motif chemokine;C-C motif chemokine 3;G0S19-1;LD78ALPHA;MIP1A;MIP-1-alpha;SCYA3
UniProt
Additional CCL3/CCL4 Products
Product Documents for Human CCL3/CCL4 Antibody
Product Specific Notices for Human CCL3/CCL4 Antibody
For research use only
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