Human CCL4/MIP-1 beta Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF271

R&D Systems, part of Bio-Techne
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BAF271
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)

Applications

Validated:

ELISA Detection (Matched Antibody Pair), Immunocytochemistry, Western Blot

Cited:

Antibody Array Development, ELISA Development, Immunocytochemistry, Immunohistochemistry-Frozen, Luminex Development, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all CCL4/MIP-1 beta Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta

Specificity

Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) MIP‑1 alpha is observed and less than 0.1% cross-reactivity with recombinant viral (rv) MIP-I, rvMIP-II, rhMIP‑1 delta, recombinant mouse (rm) MIP‑1 alpha, rmMIP‑1 beta, rmMIP-1 gamma, rhGRO beta, rhGRO gamma, rhMCP-1, and rhRANTES is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CCL4/MIP-1 beta Biotinylated Antibody

CCL4/MIP-1 beta antibody in Human PBMCs by Immunocytochemistry (ICC).

CCL4/MIP-1 beta in Human PBMCs.

CCL4/MIP-1 beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human CCL4/MIP-1 beta Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF271) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human CCL4/MIP-1 beta Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells treated with PHA

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME)

Human CCL4/MIP-1 beta Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human CCL4/MIP-1 beta Antibody (Catalog # MAB271)
  • Standard: Recombinant Human CCL4/MIP-1 beta Protein (Catalog # 271-BME)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL4/MIP-1 beta

CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta) is a 7.8 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV. Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2b. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor. Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

Alternate Names

Exodus-3, MIP-1 beta, MIP1 beta

Entrez Gene IDs

6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine); 102117861 (Cynomolgus Monkey)

Gene Symbol

CCL4

Additional CCL4/MIP-1 beta Products

Product Documents for Human CCL4/MIP-1 beta Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL4/MIP-1 beta Biotinylated Antibody

For research use only

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