Human CCL5/RANTES Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2781
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat
Applications
Validated:
Neutralization, Western Blot
Cited:
Immunohistochemistry-Paraffin, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 16411
Product Specifications
Immunogen
E. coli-derived recombinant human CCL5/RANTES
Specificity
Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human CCL1, 2, 3, 4, 7, 8,
11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, recombinant
mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20,
21, 22, 24, 25, 27, recombinant cotton rat CCL3, 4, 5, or recombinant rat
CCL20.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL5/RANTES Antibody
Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody.
Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB2781). The ND50 is typically 0.2-0.8 µg/mL.Applications for Human CCL5/RANTES Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CCL5/RANTES (Catalog # 278-RN)
Sample: Recombinant Human CCL5/RANTES (Catalog # 278-RN)
Neutralization
Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL5/RANTES.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL5/RANTES
Alternate Names
RANTES, SISd
Gene Symbol
CCL5
Additional CCL5/RANTES Products
Product Documents for Human CCL5/RANTES Antibody
Product Specific Notices for Human CCL5/RANTES Antibody
For research use only
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