Human CCL5/RANTES Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB278
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Neutralization, Western Blot
Cited:
ELISA Development, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 21445
Product Specifications
Immunogen
E. coli-derived recombinant human CCL5/RANTES
Specificity
Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with other chemokines, including recombinant human CCL2, 3, 4, recombinant mouse (rm) CCL3, and rmCCL4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL5/RANTES Antibody
Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody.
Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278). The ND50 is typically 0.1-0.4 µg/mL.
CCL5/RANTES in Mouse Small Intestine.
CCL5/RANTES was detected in perfusion fixed frozen sections of mouse small intestine using Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Human CCL5/RANTES Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse small intestine
Sample: Perfusion fixed frozen sections of mouse small intestine
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Human CCL5/RANTES transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin
Sample: Human CCL5/RANTES transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: Recombinant Human CCL5/RANTES (Catalog # 278-RN)
Sample: Recombinant Human CCL5/RANTES (Catalog # 278-RN)
Neutralization
Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL5/RANTES.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB278 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL5/RANTES
Alternate Names
RANTES, SISd
Gene Symbol
CCL5
Additional CCL5/RANTES Products
Product Documents for Human CCL5/RANTES Antibody
Product Specific Notices for Human CCL5/RANTES Antibody
For research use only
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