Human CCL8/MCP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB281
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
ELISA Development, ELISA Development (Capture), Immunocytochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 35509
Product Specifications
Immunogen
E. coli-derived recombinant human CCL8/MCP-2
Specificity
Detects human CCL8/MCP-2 in ELISAs. In ELISAs, this antibody shows less than 0.1% cross-reactivity with recombinant human (rh) CCL15 and rmCCL3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL8/MCP-2 Antibody
Chemotaxis Induced by CCL8/MCP-2 and Neutralization by Human CCL8/MCP-2 Antibody.
Recombinant Human CCL8/MCP-2 (Catalog # 281-CP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL8/MCP-2 (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL8/MCP-2 Monoclonal Antibody (Catalog # MAB281). The ND50 is typically 1-4 µg/mL.Applications for Human CCL8/MCP-2 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize CCL8/MCP-2-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.25 µg/mL Recombinant Human CCL8/MCP-2.
Human CCL8/MCP-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 3.5 using MAB281 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL8/MCP-2
Additional CCL8/MCP-2 Products
Product Documents for Human CCL8/MCP-2 Antibody
Product Specific Notices for Human CCL8/MCP-2 Antibody
For research use only
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