Human CCL8/MCP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AB-281-NA
Key Product Details
Species Reactivity
Human
Applications
Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CCL8/MCP-2
Gln24-Pro99
Accession # P80075
Gln24-Pro99
Accession # P80075
Specificity
Detects human CCL8/MCP‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) MCP-1 and rhMCP-3 is observed. Neutralizes the biological activity of recombinant human MCP-2, but will not neutralize the biological activity of MCP-1 or MCP-3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL8/MCP-2 Antibody
Chemotaxis Induced by CCL8/MCP‑2 and Neutral-ization by Human CCL8/ MCP‑2 Antibody.
Recombinant Human CCL8/MCP-2 (Catalog # 281-CP) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL8/MCP-2 (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL8/MCP-2 Polyclonal Antibody (Catalog # AB-281-NA). The ND50 is typically 60-120 µg/mL.Applications for Human CCL8/MCP-2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CCL8/MCP-2 (Catalog # 281-CP)
Sample: Recombinant Human CCL8/MCP-2 (Catalog # 281-CP)
Neutralization
Measured by its ability to neutralize CCL8/MCP-2-induced chemotaxis in human monocytes. The Neutralization Dose (ND50) is typically 60-120 µg/mL in the presence of 0.4 µg/mL Recombinant Human CCL8/MCP-2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL8/MCP-2
MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.
Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Alternate Names
MCP-2, MCP2
Gene Symbol
CCL8
UniProt
Additional CCL8/MCP-2 Products
Product Documents for Human CCL8/MCP-2 Antibody
Product Specific Notices for Human CCL8/MCP-2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...