Human CCL8/MCP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-281-NA
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Neutralization, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CCL8/MCP-2
Gln24-Pro99
Accession # P80075
Gln24-Pro99
Accession # P80075
Specificity
Detects human CCL8/MCP‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 40% cross‑reactivity with recombinant human (rh) HCC-1 is observed, less than 10% cross‑reactivity with rhEotaxin is observed, and less than 5% cross‑reactivity with rhMCP-1 is observed. Neutralizes the biological activity of rhCCL8. This antibody will not neutralize the biological activity of rhMCP-1, recombinant mouse JE, or rhMCP-3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL8/MCP-2 Antibody
Chemotaxis Induced by CCL8/MCP‑2 and Neutral-ization by Human CCL8/MCP‑2 Antibody.
Recombinant Human CCL8/MCP-2 (Catalog # 281-CP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL8/ MCP-2 (250 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL8/MCP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-281-NA). The ND50 is typically 1.5-5.0 µg/mL.Applications for Human CCL8/MCP-2 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human CCL8/MCP-2 (Catalog # 281-CP)
Sample: Recombinant Human CCL8/MCP-2 (Catalog # 281-CP)
Neutralization
Measured by its ability to neutralize CCL8/MCP-2-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 1.5-5.0 µg/mL in the presence of 250 ng/mL Recombinant Human CCL8/MCP-2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL8/MCP-2
MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.
Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Alternate Names
MCP-2, MCP2
Gene Symbol
CCL8
UniProt
Additional CCL8/MCP-2 Products
Product Documents for Human CCL8/MCP-2 Antibody
Product Specific Notices for Human CCL8/MCP-2 Antibody
For research use only
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