Human CCR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB145
Clone 53504 was used by HLDA to establish CD designation
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 53504
Product Specifications
Immunogen
Swiss 3T3 mouse cell line transfected with human CCR1
Accession # P32246
Accession # P32246
Specificity
Detects human CCR1. Reacts specifically with human CCR1 transfectants and not the parental cell line in flow cytometry.No cross‑reactivity with CCR5 transfectants is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CCR1 Antibody
Detection of CCR1 in PBMC Monocytes by Flow Cytometry
PBMC Monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR1 Monoclonal Antibody (Catalog # MAB145) or (B) isotype control antibody (Catalog # MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.Applications for Human CCR1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human whole blood monocytes
Sample: Human whole blood monocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB145 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR1
Alternate Names
CCR1, CD191
Gene Symbol
CCR1
UniProt
Additional CCR1 Products
Product Documents for Human CCR1 Antibody
Product Specific Notices for Human CCR1 Antibody
For research use only
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