Human CCR5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB180
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
ELISA Development, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 45502
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human CCR5
Met1-Leu352
Accession # P51681
Met1-Leu352
Accession # P51681
Specificity
Specifically stains human CCR5 transfectants but not the parental cell line as detected by flow cytometry. It has also been shown to react with a CCR5/CCR2 chimera (5222) containing the amino-terminal domain of CCR5. However, this antibody does not react with CCR5 present on unfixed stimulated human PBMCs by flow cytometry. For additional information regarding epitope specificity for this antibody and other R&D Systems anti-human CCR5 antibodies, see Lee, B. et al., 1999, J. Biol. Chem. 274:9617.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human CCR5 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human CCR5 transfected cells
Sample: Human CCR5 transfected cells
Reviewed Applications
Read 1 review rated 3 using MAB180 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR5
CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.
Alternate Names
CCR5, CD195
Gene Symbol
CCR5
UniProt
Additional CCR5 Products
Product Documents for Human CCR5 Antibody
Product Specific Notices for Human CCR5 Antibody
For research use only
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