Human peripheral blood mononuclear cells (PBMCs) were stained with PE-conjugated Mouse anti-Human CD4 Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197R) or (B) Mouse IgG_2Aisotype control antibody (Catalog # MAB003) followed by APC-conjugated anti-mouse IgG secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Chemotaxis Induced by CCL19/MIP-3 beta and Neutral-ization by Human CCR7 Antibody.

Recombinant Human CCL19/MIP-3 beta (Catalog # 361-MI) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197R). The ND₅₀ is typically 1-5 µg/mL.

Applications for Human CCR7 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Neutralization

Measured by its ability to neutralize CCL19/MIP‑3 beta-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR7. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 50 ng/mL Recombinant Human CCL19/MIP‑3 beta.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR7

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Alternate Names

BLR2, CC-CKR-7, CCR7, CD197, CDw197, CMKBR7, EBI1

Entrez Gene IDs

1236 (Human); 12775 (Mouse); 287673 (Rat)

Gene Symbol

CCR7

UniProt

AAA58615

Additional CCR7 Products

Product Documents for Human CCR7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CCR7 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CCR7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB197R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CCR7 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Human CCR7 Antibody

Detection of CCR7 in Human PBMCs by Flow Cytomtetry.