Human CCR8 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1429
Clone 191704 was used by HLDA to establish CD designation
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
CyTOF-ready, Flow Cytometry, Neutralization
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 191704
Product Specifications
Immunogen
BaF3 mouse pro-B cell line transfected with human CCR8
Met1-Leu355
Accession # P51685
Met1-Leu355
Accession # P51685
Specificity
Detects human CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not with the parent cell lines.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCR8 Antibody
Detection of CCR8 in Human Blood by Flow Cytometry.
Human peripheral blood cells were stained with (A) Rat Anti-Human CCR8 Monoclonal Antibody (Catalog # MAB1429) or (B) isotype control antibody (Catalog # MAB0061) followed by anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.
Chemotaxis Induced by CCL1/I-309 and Neutralization by Human CCR8 Antibody.
Recombinant Human CCL1/I-309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I-309 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR8 Monoclonal Antibody (Catalog # MAB1429). The ND50 is typically 0.01-0.05 µg/mL.Applications for Human CCR8 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood cells
Sample: Human peripheral blood cells
Neutralization
Measured by its ability to neutralize CCL1/I-309/TCA-3-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 0.01-0.05 µg/mL in the presence of 20 ng/mL Recombinant Human CCL1/I-309/TCA-3.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 1 using MAB1429 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR8
Alternate Names
CCR8, CD198, CDw198, ChemR1, CKRL1, GPRCL1, TER-1
Gene Symbol
CCR8
UniProt
Additional CCR8 Products
Product Documents for Human CCR8 Antibody
Product Specific Notices for Human CCR8 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...