Human CCR9 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1791R
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2A Clone # 248621R
Product Specifications
Immunogen
Human CCR9 transfectants
Met1-Leu369
Accession # P51686
Met1-Leu369
Accession # P51686
Specificity
Detects human CCR9 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human CCR9 Antibody
Detection of CCR9 in Human MOLT-4 cells by Flow Cytometry.
MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791R, filled histogram) or Mouse IgG2AFlow Cytometry Isotype Control (Catalog # MAB003) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR9 in HEK293 Human Cell Line Transfected with Human CCR9 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human CCR9 and eGFP was stained with (A) Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791R) or (B) Mouse IgG2Aisotype control antibody (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
CCR9 in MOLT‑4 Human Cell Line.
CCR9 was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791R) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human CCR9 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: MOLT-4 human acute lymphoblastic leukemia cell line and HEK293 Human Cell Line Transfected with Human CCR9 and eGFP
Sample: MOLT-4 human acute lymphoblastic leukemia cell line and HEK293 Human Cell Line Transfected with Human CCR9 and eGFP
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line
Sample: Immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR9
Alternate Names
CCR9, CD199, CDw199
Gene Symbol
CCR9
UniProt
Additional CCR9 Products
Product Documents for Human CCR9 Antibody
Product Specific Notices for Human CCR9 Antibody
For research use only
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