Human CD15/Lewis X Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7368
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # ICRF29-2
Product Specifications
Immunogen
human granulocytes
Specificity
Detects human CD15/Lewis X in flow cytometry-based testing.
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for Human CD15/Lewis X Antibody
Detection of CD15/Lewis X in Human Blood Granulocytes by Flow Cytometry.
Human peripheral blood granulocytes were stained with Mouse Anti-Human CD15/Lewis X Monoclonal Antibody (Catalog # MAB7368, filled histogram) or Mouse IgM control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (F0116).
CD15/Lewis X in Human Breast Cancer Tissue.
CD15/Lewis X was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue (left panel; positive staining) and normal breast (right panel; negative staining) using Mouse Anti-Human CD15/Lewis X Monoclonal Antibody (Catalog # MAB7368) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human CD15/Lewis X Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood granulocytes
Sample: Human peripheral blood granulocytes
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue and normal breast
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue and normal breast
Reviewed Applications
Read 1 review rated 5 using MAB7368 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD15/Lewis X
Alternate Names
CD15, Lewis-X, LeX, SSEA-1
Additional CD15/Lewis X Products
Product Documents for Human CD15/Lewis X Antibody
Product Specific Notices for Human CD15/Lewis X Antibody
For research use only
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