Human CD157 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4736
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
ELISA Development (Capture), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD157
Gly29-Lys292
Accession # Q10588
Gly29-Lys292
Accession # Q10588
Specificity
Detects human CD157 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CD157 Antibody
Detection of Human CD157 by Western Blot.
Western blot shows lysates of human kidney (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD157 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4736) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD157 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human CD157 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human kidney (cortex) tissue
Sample: Human kidney (cortex) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD157
CD157, also known as bone marrow stromal cell antigen 1 (BST-1), is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family (1). CD157 was discovered in a bone marrow stromal cell line where it facilitates pre-B-cell growth (2, 3). Along with CD38, CD157 is a bifunctional ectoenzyme that exhibits both ADP-ribosyl cyclase and cyclic ADP ribose hydrolase activities (2). It may play a role in rheumatoid arthritis (RA) due to its enhanced expression in RA‑derived bone marrow stromal cell lines (3). CD157 has been predicted to function as a cell surface receptor and an immunoregulatory molecule (4).
References
- Hussain, A. M. M. et al. (1998) Protein Express. Purif. 12:133.
- Sato, A. et al. (1999) Biochem. J. 337:491.
- Kaisho, T. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5325.
- Ortolan, E. et al. (2002) Cell Biochem. Funct. 20:309.
Long Name
Cluster of Differentiation 157
Alternate Names
BP-3, BST1, cADPr hydrolase 2, CD157
Gene Symbol
BST1
UniProt
Additional CD157 Products
Product Documents for Human CD157 Antibody
Product Specific Notices for Human CD157 Antibody
For research use only
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