Human CD164 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5790
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD164 extracellular domain
Asp24-Leu197
Accession # Q04900
Asp24-Leu197
Accession # Q04900
Specificity
Detects human CD164 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD164 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CD164 Antibody
Detection of Human CD164 by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5790) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD164 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2Applications for Human CD164 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: U937 human histiocytic lymphoma cell line and human peripheral blood mononuclear cells (PBMC)
Sample: U937 human histiocytic lymphoma cell line and human peripheral blood mononuclear cells (PBMC)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD164
Alternate Names
CD164, MGC-24, MUC-24
Gene Symbol
CD164
UniProt
Additional CD164 Products
Product Documents for Human CD164 Antibody
Product Specific Notices for Human CD164 Antibody
For research use only
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