Detects human CD1a in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CD1d is observed and no cross-reactivity with with rhCD1b or rhCD1e is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human CD1a APC-conjugated Antibody

Detection of CD1a in MOLT‑4 Human Cell Line by Flow Cytometry.

MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CD1a APC-conjugated Monoclonal Antibody (Catalog # FAB7076A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD1a APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD1a

CD1a (also known as Leu6) ia a 48-50 kDa member of the five gene CD1 family of molecules. It is a type I transmembrane glycoprotein that is 311 amino acids (aa) in length. It contains three " alpha-domains" in its extracellular region (aa 17-300), and terminates with a short six aa segment. Although CD1a orthologs are found in multiple mammalian species, it does not appear to exist in rodent. Notably, not all humans possess a functional CD1a gene. CD1a serves as an MHC Class I type molecule that presents lipids to alpha beta TCRs on T cells. This means it exists as a heterodimer with beta2-microglobulin on the cell surface. CD1a is unique in that it can present nonhydrophilic lipids to T cells; that is, there is no need for a polar "head" on one end of the lipid to interact with the polar residues of the TCR protein. Other cell membrane components suggested to associate with CD1a include CD74 and CD9, two molecules that may regulate cycling of CD1a. Cells that are known to express CD1a are restricted in number, and include cortical thymocytes, Langerhans cells, dermal dendritic cells (DC), and monocyte-derived DC. It has been suggested that type I mono-DCs, or those that secrete IL-12 and promote Th1 formation, are uniquely CD1a+. At this time, this appears to be too restrictive an assumption, and that the presence or absence of CD1a is only generally reflective of function.

Long Name

T Cell Surface Glycoprotein CD1a

Alternate Names

CD1a, FCB6, HTA1, R4, T6

Entrez Gene IDs

909 (Human)

Gene Symbol

CD1A

UniProt

P06126

Additional CD1a Products

Product Documents for Human CD1a APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human CD1a APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human CD1a APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7076A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD1a Antibodies »

Product Specifications

Immunogen

Specificity