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Human CD1b Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7446G

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 737249

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD1b
Ser18-Ser303
Accession # P29016

Specificity

Detects human CD1b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1c, rhCD1d, rhCD1e, recombinant mouse (rm) CD1d1, or rmCD1d2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD1b Alexa Fluor® 488-conjugated Antibody

Detection of CD1b antibody in MOLT-4 Human Cell Line antibody by Flow Cytometry.

Detection of CD1b in MOLT‑4 Human Cell Line by Flow Cytometry.

MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CD1b Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7446G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD1b Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD1b

CD1b is a 44-46 kDa member of the CD1 family of proteins. It is one of five CD1 isoforms (CD1a-e) that resemble MHC-I type molecules. Based on structure, CD1a, 1b and 1c can be grouped together, with CD1d and 1e existing as stand-alone forms. CD1b is a 316 amino acid (aa) type I transmembrane glycoprotein that contains one Ig-like domain in its ECD. It is expressed in the late endosome/lysosome compartment of myeloid-type dendritic cells. Following synthesis, CD1b associates with SAP-C, binds two short lipids in an unusually large ligand-binding groove, and transits either to the plasma membrane, or more commonly, the endosomal compartment. In either case, exposure to Gm- lipid A or mycobacterial glyolipid results in a substitution of the foreign lipid for the two short-chain self lipids, followed by its presentation on the plasma membrane. Select alpha beta and gamma delta T cell recognize these lipids and typically repond with Th1-type cytokines (IFN-gamma and TNF-alpha). Unlike other CD1 family molecules, CD1b does not seem to have a soluble form. There is no rodent counterpart to human CD1b, only to CD1d.

Alternate Names

CD1b

Entrez Gene IDs

910 (Human)

Gene Symbol

CD1B

UniProt

Additional CD1b Products

Product Documents for Human CD1b Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD1b Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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