HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP was stained with and either (A) Mouse Anti-Human MS4A1/CD20 PE-conjugated Monoclonal Antibody (Catalog # FAB4225P) or (B) Mouse IgG₁Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Detection of MS4A1/CD20 in Human Peripheral Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human MS4A1/CD20 PE-conjugated Monoclonal Antibody (Catalog # FAB4225P) or (B) Mouse IgG₁Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD20 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes and HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD20

CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanning protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa identity between human and mouse CD20.

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Gene Symbol

MS4A1

UniProt

P11836

Additional CD20 Products

Product Documents for Human CD20 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD20 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human CD20 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4225P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD20 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD20 PE-conjugated Antibody

Detection of MS4A1/CD20 in HEK293 Human Cell Line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry.