Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB9575B
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry
Label
Biotin
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu2
Product Specifications
Immunogen
Human CD20
Specificity
Detects Human CD20 based on Rituximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Rituximab.
This
product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Scientific Data Images
Detection of MS4A1/CD20 in HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry.
HEK293 Human cell line transfected with (A) Human MS4A1/CD20 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human MS4A1/CD20 (Rituximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9575B) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.
CD20 in Daudi Human Cell Line.
CD20 was detected in unfixed live Daudi human Burkitt's lymphoma cell line using Human Anti-Human CD20 (Research Grade Rituximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9575B) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP
Sample: HEK293 Human cell line Transfected with Human MS4A1/CD20 and eGFP
Immunocytochemistry
8-25 µg/mL
Sample: Unfixed live Daudi human Burkitt's lymphoma cell line
Sample: Unfixed live Daudi human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD20
Long Name
Cluster of Differentiation 20
Alternate Names
B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7
Entrez Gene IDs
Gene Symbol
MS4A1
Additional CD20 Products
Product Specific Notices
Rituximab is a biosimliar expressed in HEK293 cells and is intended for research use only.
For research use only
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