Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human CD25/IL-2R alpha Antibody

IL-2 binding to Human CD25/IL-2R alpha-transfected HEK293 Human Cell Line is Blocked by Human CD25/IL-2R alpha Antibody.

In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (Catalog # BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Mouse Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB1020). Mouse IgG2A Isotype Control (Catalog # MAB003) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050).

Applications for Human CD25/IL-2R alpha Antibody

Application

Recommended Usage

Blockade of Receptor-ligand Interaction

0.5 µg/10⁶ cells
Sample: In a functional flow cytometry test, 0.5 μg/mL of Mouse Anti-Human CD25/IL-2R alpha Antibody(Catalog # MAB1020) will block the binding of 500 ng/mL Biotinylated Recombinant Human IL-2 (Catalog # BT202) to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human Treg cells (PBMC treated overnight with anti-human CD3 (10 μg/mL; Catalog # MAB100), anti-human CD28 (5 μg/mL; Catalog # MAB342), TGF-beta (10 ng/mL; Catalog # 100-B), and rhIL-2 (20 ng/mL; Catalog # 202-IL).

Western Blot

1 µg/mL
Sample: Recombinant Human CD25/IL‑2 R alpha Fc Chimera (Catalog # 1020-RL)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Gene Symbol

IL2RA

UniProt

P01589

Additional CD25/IL-2R alpha Products

Product Documents for Human CD25/IL-2R alpha Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD25/IL-2R alpha Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD25/IL-2R alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1020

Key Product Details

Species Reactivity

Validated:

Cited:

Applications