Human CD25/IL-2R alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB623
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Xenograft
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Antibody Array Development, ELISA Development, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 24204
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
Glu22-Cys213
Accession # P01589
Glu22-Cys213
Accession # P01589
Specificity
Detects human CD25/IL-2 R alpha in ELISAs and Western blots. In Western blots, detects recombinant rat CD25/IL-2 R alpha but no cross-reactivity with recombinant mouse CD25/IL-2 R alpha. In sandwich immunoassays, this antibody does not cross-react or interfere with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-2 R beta, rhIL-3 R alpha, rhIL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-6 R, or rhgp130.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD25/IL-2R alpha Antibody
Detection of Recombinant Human and Rat CD25/IL-2 R alpha by Western Blot.
Western blot shows 25 ng of Recombinant Human CD25/IL-2 R alpha (Catalog # 223-2A), Recombinant Mouse CD25/IL-2 R alpha (Catalog # 2438-RM) and Recombinant Rat CD25/IL-2 R alpha Fc Chimera (Catalog # 5156-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB623) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD25/IL-2 R alpha at approximately 30-250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.
CD25/IL‑2 R alpha in Human PBMCs.
CD25/IL-2 Ra was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB623) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Upper panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD25/IL‑2 R alpha in Human Lymph Node.
CD25/IL‑2 R alpha was detected in immersion fixed frozen sections of human lymph node using 15 µg/mL Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB623) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Human CD25/IL-2R alpha Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells stimulated with PHA
Sample: Immersion fixed human peripheral blood mononuclear cells stimulated with PHA
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of human lymph node
Sample: Immersion fixed frozen sections of human lymph node
Western Blot
1 µg/mL
Sample: Recombinant Human CD25/IL‑2 R alpha (Catalog # 223-2A) under non-reducing conditions only
Sample: Recombinant Human CD25/IL‑2 R alpha (Catalog # 223-2A) under non-reducing conditions only
Human CD25/IL-2 R alpha Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB623 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD25/IL-2R alpha
Long Name
Interleukin 2 Receptor alpha
Alternate Names
CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA
Entrez Gene IDs
Gene Symbol
IL2RA
UniProt
Additional CD25/IL-2R alpha Products
Product Documents for Human CD25/IL-2R alpha Antibody
Product Specific Notices for Human CD25/IL-2R alpha Antibody
For research use only
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