THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD27 Ligand/TNFSF7 Monoclonal Antibody (Catalog # MAB27381, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Goat anti-mouse PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Detection of CD27Ligand/TNFSF7 in HEK293 Human Cell Line Transfected with Human CD27 Ligand/TNFSF7 by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human CD27 Ligand/TNFSF7 (filled histogram) or irrelevant protein (open histogram) was stained with Mouse Anti-Human CD27 Ligand/TNFSF7 Monoclonal Antibody (Catalog # MAB27381) followed by Goat anti-mouse PE-conjugated secondary antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human CD27 Ligand/TNFSF7 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: THP-1 Human Cell Line and HEK293 human embryonic kidney cell line transfected with human CD27 Ligand/TNFSF7

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27 Ligand/TNFSF7

Human CD27 Ligand (also CD70 and TNFSF7) is a 30 kDa type 2 transmembrane glycoprotein that is a member of the TNF superfamily. It contains a 20 amino acid (aa) cytoplasmic region and a 155 aa extracellular domain that shows multiple beta-strands. It is an inducible trimer that occurs on B cells, T cells, and NK cells. It regulates T cell dependant B cell differentiation into plasma cells and likely to promotes clonal expansion of T cells. Human CD27 Ligand extracellular region is 64% aa identical to mouse and rat CD27 Ligand extracellular regions.

Alternate Names

CD70, TNFSF7

Entrez Gene IDs

970 (Human); 21948 (Mouse); 301132 (Rat)

Gene Symbol

CD70

UniProt

P32970

Additional CD27 Ligand/TNFSF7 Products

Product Documents for Human CD27 Ligand/TNFSF7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD27 Ligand/TNFSF7 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD27 Ligand/TNFSF7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB27381

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD27 Ligand/TNFSF7 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD27 Ligand/TNFSF7 Antibody

Detection of CD27Ligand/TNFSF7 in THP-1 Human Cell Line by Flow Cytometry.