Skip to main content

Human CD27/TNFRSF7 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB382G

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 57703

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD27
Thr21-Ile192
Accession # P26842

Specificity

Detects human CD27 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) 4‑1BB, rhBAFF R, recombinant mouse (rm) CD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rmOX40, rhRANK, rhTAJ, or rhTNF RI is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD27/TNFRSF7 Alexa Fluor® 488-conjugated Antibody

Detection of CD27/TNFRSF7 in PBMC lymphocytes by Flow Cytometry

PBMC lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD27/TNFRSF7 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB382G) or (B) isotype control antibody (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD27/TNFRSF7 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human whole blood lymphocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD27/TNFRSF7

Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is up-regulated after TCR stimulation. Within the CD4+ compartment, it is preferentially expressed on CD45RA+ cells. In contrast, it is preferentially expressed on CD45RO+ cells in the CD8+ compartment. CD27 also appears to be a potential marker for memory B cells. It exists as both a disulfide‑linked dimer on the cell surface and as a soluble protein found in serum. Human CD27 is a 260 amino acid (aa) protein with a 20 aa signal, a 173 aa extracellular domain, a 20 aa transmembrane domain, and a 47 aa cytoplasmic domain. The ligand for CD27 is CD70. CD70 is expressed on thymic stromal cells and a small subset of activated T cells. Additionally a subset of activated B cells express CD70. The CD27/CD70 interaction appears to be a weak costimulatory pathway involved in T cell and B cell immune response. CD27/CD70 interactions may be more involved in controlling the expansion phase of an immune response. This would be in contrast to B7/CD28 interactions, which are important for the activation phase of immune responses.

References

  1. Camerini, D. et al. (1991) J. Immunol. 147:3165.
  2. Loenen, W.A. et al. (1992) J. Immunol. 149:3937.
  3. Lens, S.M.A. et al. (1998) Sem. Immunol. 10:491.

Alternate Names

CD27, TNFRSF7

Entrez Gene IDs

939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

Gene Symbol

CD27

UniProt

Additional CD27/TNFRSF7 Products

Product Documents for Human CD27/TNFRSF7 Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD27/TNFRSF7 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Loading...
Loading...
Loading...
Loading...
Loading...