Human CD3 GMP Antibody GMP

R&D Systems, part of Bio-Techne | Catalog # MAB11411-GMP

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

T Cell Stimulation

Label

Unconjugated

Antibody Source

Monoclonal Human IgG1 Clone # 28497-1

View all CD3 Antibodies »

Product Specifications

Immunogen

Human T lymphocytes

Specificity

Detects human CD3 in a flow cytometry-based assay using Jurkat cells

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Purity

≥95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Host Cell Protein

≤0.500 ng per μg of antibody when tested by ELISA

Host Cell DNA

≤0.0100 ng per μg of antibody when tested by PCR

Mycoplasma

Negative when tested in a ribosomal RNA hybridization assay

Activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909.
The ED50 for this effect is 8.00‑80.0 ng/mL

Aggregation

≤10% aggregation when tested by SEC

Scientific Data Images for Human CD3 GMP Antibody

Recombinant Human CD3 GMP Antibody Stimulates IL-2 Secretion in Jurkat Cells.

Human CD3 GMP antibody stimulates IL-2 secretion in Jurkat cells (immortalized human T cell line) treated with 5 ng/mL phorbol myristate acetate (PMA). Antibody-mediated stimulation occurred in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA kit. The ED50 for this effect is 8.00-80.0 ng/mL.

Applications for Human CD3 GMP Antibody

Application
Recommended Usage

T Cell Stimulation

1 µg/mL
Sample: Human PBMCs

Formulation, Preparation, and Storage

Purification

Protein G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD3

The Bio-Techne CD3 GMP antibody is derived from the OKT3 clone and binds specifically to the epsilon subunit of the CD3 complex.  CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is critical for the activation of T cells.  Specifically, the CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta.  These CD3 subunits are structurally related members of the immunoglobulin super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits.  The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum.

Alternate Names

CD_antigen: CD3e, CD3 antigen, delta subunit, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3-DELTA, CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3-epsilon, CD3G, CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD3-GAMMA, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD18, MGC138597, T3DOKT3, delta chain, T3E, T-cell antigen receptor complex, epsilon subunit of T3, T-cell receptor T3 delta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, TCRE

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Gene Symbol

CD3E

Additional CD3 Products

Product Documents for Human CD3 GMP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Manufacturing Specifications

GMP Antibodies
R&D Systems, a Bio-Techne Brand's GMP Antibodies are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products, and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.
R&D Systems' quality focus includes:
  • Manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
  • Documented processes and QA control of documentation and process changes
  • Personnel training programs
  • Raw material inspection and supplier qualification/monitoring
  • Fully validated equipment, processes and test methods
  • Equipment calibration monitored and scheduled using a computerized calibration program
  • Facility maintenance, safety programs and pest control
  • Variances dispositioned using material review process
  • Robust product stability program

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their research. The Certificate of Analysis provided contains the following lot specific information:
  • Purity (SDS-PAGE)
  • Aggregation (SEC)
  • Endotoxin (LAL method, tested per USP<85> and Ph. Eur. 2.6.14 guidelines)
  • Residual Host Cell Protein and Host Cell DNA
  • Mycoplasma testing
  • Post-bottling lot-specific bioassay results (compliance with an established range) and results of microbial testing according to USP <71>

Additional testing and documentation requested by the customer can be arranged at an additional cost.
Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis, Minnesota USA.
R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.

Product Specific Notices for Human CD3 GMP Antibody

END USER TERMS OF USE OF PRODUCT

The following terms are offered to you upon your acceptance of these End User Terms of Use of Product. By using this product, you indicate your acknowledgment and agreement to these End User Terms of Use of Product. If you do not agree to be bound by and comply with all of the provisions of these End User Terms of Use of Product, you should contact your supplier of the product and make arrangements to return the product.

We suggest you print and retain a copy of these End User Terms of Use of Product for your records.

The End User is aware that R&D Systems, Inc. sells GMP products for preclinical or clinical ex vivo use and not for in vivo use. The End User further agrees, as a condition of the sale of R&D Systems' GMP products that: a) the End User will not use this GMP Product in any procedure wherein the product may be directly or indirectly administered to humans, unless the End User has obtained, or prior to their use will have obtained, an Investigational New Drug (IND) exemption from the FDA and will use the product only in accordance with the protocols of such IND and of the Institutional Review Board overseeing the proposed research, or b) the End User will use the products outside of the United States in accordance with the protocols of research approved by the Institutional Review Board or authorized ethics committee and regulatory agencies to which the End User is subject to in their territory.

R&D Systems, Inc. has the right, at its sole discretion, to modify, add or remove any terms or conditions of these End User Terms of Use without notice or liability to you. Any changes to these End User Terms of Use are effective immediately following the printing of such changes on this product insert. The most recent version of these End User Terms of Use of Product may be found at: bio-techne.com/terms-and-conditions.

You agree to review these End User Terms of Use of Product to ensure any subsequent use by you of R&D Systems' GMP Products following changes to these End User Terms of Use of Product constitutes your acceptance of all such changes.

 

TERMS AND CONDITIONS

The following limitation applies to R&D Systems' warranty and liability for damages: All products are warranted to meet R&D Systems' published specifications when used under normal laboratory conditions.

R&D SYSTEMS DOES NOT MAKE ANY OTHER WARRANTY OR REPRESENTATION WHATSOEVER, WHETHER EXPRESS OR IMPLIED, WITH RESPECT TO ITS PRODUCTS. IN PARTICULAR, R&D SYSTEMS DOES NOT MAKE ANY WARRANTY OF SUITABILITY, NONINFRINGEMENT, MERCHANTABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE.

NOTWITHSTANDING ANY OTHER PROVISIONS OF THESE TERMS AND/OR ANY OTHER AGREEMENT BETWEEN R&D SYSTEMS AND PURCHASER FOR THE PURCHASE OF THE PRODUCTS, R&D SYSTEMS' TOTAL LIABILITY TO PURCHASER ARISING FROM OR IN RELATION TO THESE TERMS, AN AGREEMENT BETWEEN THE PARTIES OR THE PRODUCTS, WHETHER ARISING IN CONTRACT, TORT OR OTHERWISE SHALL BE LIMITED TO THE TOTAL AMOUNT PAID BY PURCHASER TO R&D SYSTEMS FOR THE APPLICABLE PRODUCTS. IN NO EVENT WILL R&D SYSTEMS BE LIABLE FOR THE COST OF PROCUREMENT OF SUBSTITUTE GOODS.

The most recent version of these End User Terms of of Product may be found at: bio-techne.com/terms-and-conditions.

For research use only

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