Human CD30/TNFRSF8 (Research Grade Brentuximab Biosimilar) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9576

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu8

View all CD30/TNFRSF8 Antibodies »

Product Specifications

Immunogen

Human CD30/TNFRSF8

Specificity

Detects human CD30/TNFRSF8 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Brentuximab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CD30/TNFRSF8 (Research Grade Brentuximab Biosimilar) Antibody

Detection of CD30/TNFRSF8 antibody in Jurkat human acute T cell leukemia cell line antibody by Flow Cytometry.

Detection of CD30/TNFRSF8 in Jurkat human acute T cell leukemia cell line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Human Anti-Human CD30/TNFRSF8 (Brentuximab Biosimilar) Monoclonal Antibody (Catalog # MAB9576, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD30/TNFRSF8 (Research Grade Brentuximab Biosimilar) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD30/TNFRSF8

Brentuximab is a biosimilar directed against the extracellular domain of CD30. This product constitutes the immunogen used for the anti-Idiotype catalog number MAB9584. The biotinylated antibody (catalog number FAB9576B) can be used in flow cytometry or various assay format to measure CD30 expression and can be detected by conjugated streptavidin.

Alternate Names

CD30, TNFRSF8

Entrez Gene IDs

943 (Human); 21941 (Mouse); 25069 (Rat)

Gene Symbol

TNFRSF8

Additional CD30/TNFRSF8 Products

Product Documents for Human CD30/TNFRSF8 (Research Grade Brentuximab Biosimilar) Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD30/TNFRSF8 (Research Grade Brentuximab Biosimilar) Antibody

For research use only

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