Human CD300g/Nepmucin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5328
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD300g/Nepmucin
Leu19-Leu249
Accession # Q6UXG3
Leu19-Leu249
Accession # Q6UXG3
Specificity
Detects human CD300g/Nepmucin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD300g/Nepmucin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CD300g/Nepmucin Antibody
Detection of Human CD300g/Nepmucin by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD300g/Nepmucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5328) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD300g/Nepmucin at approximately 75-95 kDa and 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human CD300g/Nepmucin Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and THP‑1 human acute monocytic leukemia cell line
Sample: HepG2 human hepatocellular carcinoma cell line and THP‑1 human acute monocytic leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD300g/Nepmucin
Nepmucin (A mucin not expressed in Peyer's Patches; also CD300g, CLM9, and TREM4) is a 75-95 kDa, O-glycosylated member of the CD300 family of molecules. It is expressed on capillary endothelium, and appears to serve multiple functions. Via a mucin domain, it binds lymphocyte L-selectin, contributing to cell migration. It also binds immunoglobulins A and M, perhaps mediating transendothelial transport. Mature human nepmucin is a 314 amino acid (aa ) type I transmembrane glycoprotein. It contains one V-type Ig-like domain (aa 19-121), a mucin stalk (aa 137-237), and a cytoplasmic region (aa 269-332). There are at least two isoform variants of nepmucin. Each shows a deletion of aa 128-212, accompanied by either a 23 aa, or 12 aa substitution for aa 296-332. Over aa 19-249, human nepmucin shares 51% aa identity with mouse nepmucin.
Alternate Names
CD300LG, CLM9, Nepmucin, TREM-4
Gene Symbol
CD300LG
UniProt
Additional CD300g/Nepmucin Products
Product Documents for Human CD300g/Nepmucin Antibody
Product Specific Notices for Human CD300g/Nepmucin Antibody
For research use only
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