Human CD34 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB72274

R&D Systems, part of Bio-Techne
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MAB72274-100
MAB72274-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1054015

View all CD34 Antibodies »

Product Summary for Human CD34 Antibody

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human CD34 protein
Ser32-Thr290
Accession # P28906-1

Specificity

Detects human CD34 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD34 Antibody

Detection of CD34 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB72274) or (B) Mouse IgG2B isotype control antibody (MAB0041) followed by APC-conjugated Anti-Mouse IgG secondary antibody and Mouse Anti-Human CD45 PE-conjugated Monoclonal Antibody (FAB1430P). View our protocol for Staining Membrane-associated Proteins.

Detection of CD34 in KG-1 (positive) and K562 (negative) cells.

CD34 was detected in immersion fixed KG-1 (positive) and absent in K562 (negative) cells using Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB72274) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of CD34 in KG-1 cells by Flow Cytometry.

KG-1 cells were stained with Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB72274, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD34 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMCs and KG‑1 human acute myelogenous leukemia cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed KG-1 (positive) and K562 (negative) cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD34

CD34 is a 115 kDa glycosylated type I transmembrane protein; it was discovered as a hematopoietic cell-surface antigen (1, 2, 3). Human CD33 cDNA encodes a 385 amino acid (aa) precursor that contains a 31 aa signal sequence, a 259 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 74 aa cytoplasmic domain. Within the ECD, human CD34 shares 55% and 52% aa sequence identity with mouse and rat CD34, respectively. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC) (4, 5). CD34 is found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines, and it is a common marker for diverse progenitors (6). CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

References

  1. Civin C.I. et al. (1984) J. Immunol. 133:157.
  2. Katz F. et al. (1985) Leuk. Res. 9:191.
  3. Andrews R.G. et al. (1986) Blood 67:842.
  4. Young P.E. et al. (1995) Blood 85:96.
  5. Krause D.S. et al. (1996) Blood 87:1.
  6. Sidney L.E. et al. (2014) Stem Cells 32:1380.

Alternate Names

CD34, HPCA1

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Gene Symbol

CD34

UniProt

P28906-1

Additional CD34 Products

Product Documents for Human CD34 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD34 Antibody

For research use only

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