Human CD39L1/ENTPD2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6087
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD39L1/ENTPD2
Thr29-Asp460
Accession # Q9Y5L3
Thr29-Asp460
Accession # Q9Y5L3
Specificity
Detects human CD39L1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse CD39L1 and less than 1% cross-reactivity with recombinant human (rh) CD39L2, rhCD39L3, and rhCD39L4 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CD39L1/ENTPD2 Antibody
Detection of Human CD39L1/ENTPD2 by Western Blot.
Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Human CD39L1/ENTPD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6087) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD39L1/ENTPD2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human CD39L1/ENTPD2 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human CD39L1/ENTPD2 (Catalog # 6087-EN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human CD39L1/ENTPD2 (Catalog # 6087-EN), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Human heart tissue
Sample: Human heart tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD39L1/ENTPD2
CD39L1, also known as ENTPD2 and NTPDase2, is an ectonucleotidase belonging to the CD39 family. It is found on the surface of vascular adventitial cells and accessory vascular cells (1). CD39L1 is a Ca2+ and Mg2+ dependent enzyme that activates platelets by preferentially converting ATP to ADP (2). CD39L1 plays a role in regulating thrombosis and inflammation (3). It is considered to be a therapeutic target for thromboregulation and the treatment of vascular inflammation (2, 4).
References
Zimmermann, H. et al. 2000 Proceedings of the Second International Workshop on EctoATPases and Related Ectonucleotidases:18.
Robson, S.C. et al. 2001 Drug Dev. Res. 53:193.
Marcus, A.J. et al. 2005 Semin. Thromb. Hemost. 31:234.
Sevigny, J. et al. 2002 Blood 99:2801.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 2
Alternate Names
CD39L1, Ecto-ATPase, ENTPD2, NTPD2, NTPDase 2
Gene Symbol
ENTPD2
UniProt
Additional CD39L1/ENTPD2 Products
Product Documents for Human CD39L1/ENTPD2 Antibody
Product Specific Notices for Human CD39L1/ENTPD2 Antibody
For research use only
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