Human CD4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11426

R&D Systems, part of Bio-Techne
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MAB11426-100
MAB11426-SP
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Key Product Details

Species Reactivity

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1068819

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Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human CD4 
Lys26-Trp390
Accession # P01730

Specificity

Detects human CD4 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD4 Antibody

Detection of Human CD4 by Simple WesternTM.

Simple Western lane view shows lysates of Human PBMCs, loaded at 0.2 mg/mL. A specific band was detected for CD4 at approximately 65 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB11426) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human CD4 by Western Blot.

Western blot shows lysates of PBMC, SUP-T1 human T cell lymphoblastic lymphoma cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB11426) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human CD4 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Human PBMCs

Western Blot

2 µg/mL
Sample: PBMC, SUP-T1 human T cell lymphoblastic lymphoma cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations (1). Mature human CD4 consists of a 371 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain (2). Within the ECD, human CD4 shares approximately 52% aa sequence identity with mouse and rat CD4. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells (3). CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (4). CD4 is reexpressed on circulating CD8+ T cells upon activation and contributes to their cytotoxic effector activity (5). In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain (6-9). Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse (6). CD4 binds directly to MHC class II molecules on antigen presenting cells (10). This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction (1, 11). Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck (12). CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1 (7, 13-15).

References

  1. Vignali, D.A.A. (2010) J. Immunol. 184:5933.
  2. Maddon, P.J. et al. (1985) Cell 42:93.
  3. Alarcon, B. and H.M. van Santen (2010) Sci. Signal. 3:pe11.
  4. Wan, Y.Y. and R.A. Flavell (2009) Mol. Cell Biol. 1:20.
  5. Kitchen, S.G. et al. (2005) Proc. Natl. Acad. Sci. 102:3794.
  6. Crocker, P.R. et al. (1987) J. Exp. Med. 166:613.
  7. Biswas, P. et al. (2003) Blood 101:4452.
  8. Bernstein, H.B. et al. (2006) J. Immunol. 177:3669.
  9. Funke, I. et al. (1987) J. Exp. Med. 165:1230.
  10. Doyle, C. and J.L. Strominger (1987) Nature 330:256.
  11. Huppa, J.B. et al. (2010) Nature 463:963.
  12. Fragoso, R. et al. (2003) J. Immunol. 170:913.
  13. Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. 91:5109.
  14. Klatzmann, D. et al. (1984) Nature 312:767.
  15. Dagleish, A.G. et al. (1984) Nature 312:763.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

UniProt

P01730

Additional CD4 Products

Product Documents for Human CD4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD4 Antibody

For research use only

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