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Human CD4 mFluor™ Violet 500-Conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3791MFV500

R&D Systems, part of Bio-Techne
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FAB3791MFV500-100

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 11830

Product Specifications

Immunogen

Recombinant Human CD4.
Extracellular Domain
Accession # P01730

Specificity

Detects human CD4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD4 mFluor™ Violet 500-Conjugated Antibody

Detection of CD4 in PBMCs by Flow Cytometry.

PBMCs were stained with Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD4 mFluor™ Violet 500-Conjugated Monoclonal Antibody (Catalog # FAB3791MFV500) or (B) unstained cells. View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD4 mFluor™ Violet 500-Conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: PBMCs with CD3 costain
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

UniProt

Additional CD4 Products

Product Documents for Human CD4 mFluor™ Violet 500-Conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD4 mFluor™ Violet 500-Conjugated Antibody

mFluorTM is a trademark of AAT Bioquest, Inc.

For research use only

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