We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?

Detects human CD40/TNFRSF5 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rhRANK, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse CD40.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human CD40/TNFRSF5 PE-conjugated Antibody

Detection of CD40/TNFRSF5 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD40/TNFRSF5 PE-conjugated Monoclonal Antibody (Catalog # FAB6321P) and Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F). Quadrant markers were set based on control antibody staining (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.

Detection of CD40/TNFRSF5 in PBMC lymphocytes by Flow Cytometry

PBMC lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD40/TNFRSF5 PE-conjugated Monoclonal Antibody (Catalog # FAB6321P) or (B) isotype control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD40/TNFRSF5 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a 45-50 kD type I transmembrane glycoprotein that belongs to the TNF receptor superfamily. Mature human CD40 is 257 amino acids (aa) in length, and consists of a 173 aa extracellular domain (ECD), a 22 aa transmembrane segment and a 62 aa cytoplasmic region. The ECD contains four cysteine-rich repeats, the second of which is considered crucial to ligand binding. Over aa 21-193, human CD40 shares 58% aa sequence identity with mouse CD40. Although not ubiquitously expressed, cells known to constitutively or inducibly express CD40 are large in number and include basal layer keratinocytes, adipocytes, CD8⁺ T cells, fibrocytes (circulating stem cells), monocytes, eosinophils, dendritic cells, pancreatic duct epithelium, astrocytes, smooth muscle cells, fibroblasts, vascular endothelial cells and B cells. There is at least one alternative splice form that shows a 38 aa substitution for aa 166-277, generating a soluble molecule. There are two "ligands" for CD40, the first being CD154/CD40L, and the second Borrelia, a spirochete that causes Lyme disease. CD40 ligation by CD154 has many outcomes, some of which are cell-specific. Ligation of CD40 on B cells (with IL-4) induces IgE production, while bone marrow stromal cell activation via CD40 promotes OPG secretion with concomitant bone formation. In addition, stimulation of CD40 on keratinocytes results in IL-8 and RANTES expression with subsequent leukocyte infiltration, while adipocyte activation via CD40 promotes local M1 polarization.

Alternate Names

CD40, TNFRSF5

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)

Gene Symbol

CD40

UniProt

P25942

Additional CD40/TNFRSF5 Products

Product Documents for Human CD40/TNFRSF5 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human CD40/TNFRSF5 PE-conjugated Antibody

For research use only

Citations
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Protocols
FAQs

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Flow Cytometry

Human CD40/TNFRSF5 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6321P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications